Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator
Identifieur interne : 003A23 ( Main/Exploration ); précédent : 003A22; suivant : 003A24Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator
Auteurs : David R. Russell [États-Unis] ; George N. Bennett [États-Unis]Source :
- Gene [ 0378-1119 ] ; 1981.
Abstract
A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.
Url:
DOI: 10.1016/0378-1119(82)90096-8
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000C52
- to stream Istex, to step Curation: 000C52
- to stream Istex, to step Checkpoint: 001673
- to stream Main, to step Merge: 003B89
- to stream Main, to step Curation: 003A23
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title><author><name sortKey="Russell, David R" sort="Russell, David R" uniqKey="Russell D" first="David R." last="Russell">David R. Russell</name></author><author><name sortKey="Bennett, George N" sort="Bennett, George N" uniqKey="Bennett G" first="George N." last="Bennett">George N. Bennett</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno><date when="1982" year="1982">1982</date><idno type="doi">10.1016/0378-1119(82)90096-8</idno><idno type="url">https://api.istex.fr/document/7B3E2C0AC7EACC67BF7C383593CF127FE124EB89/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000C52</idno><idno type="wicri:Area/Istex/Curation">000C52</idno><idno type="wicri:Area/Istex/Checkpoint">001673</idno><idno type="wicri:doubleKey">0378-1119:1982:Russell D:cloning:of:small</idno><idno type="wicri:Area/Main/Merge">003B89</idno><idno type="wicri:Area/Main/Curation">003A23</idno><idno type="wicri:Area/Main/Exploration">003A23</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator</title><author><name sortKey="Russell, David R" sort="Russell, David R" uniqKey="Russell D" first="David R." last="Russell">David R. Russell</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Department of Biochemistry, Rice University, Houston</wicri:cityArea></affiliation></author><author><name sortKey="Bennett, George N" sort="Bennett, George N" uniqKey="Bennett G" first="George N." last="Bennett">George N. Bennett</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Department of Biochemistry, Rice University, Houston</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">17</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="9">9</biblScope><biblScope unit="page" to="18">18</biblScope></imprint><idno type="ISSN">0378-1119</idno></series><idno type="istex">7B3E2C0AC7EACC67BF7C383593CF127FE124EB89</idno><idno type="DOI">10.1016/0378-1119(82)90096-8</idno><idno type="PII">0378-1119(82)90096-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A 41-bp AluI restriction fragment from the trp promoter-operator region has been cloned into the PvuII site of pBR322, regenerating PvuII sites. Transformants were selected on media that allowed the selection of trp-operator-bearing plasmids. The cloned 41-bp fragment can be released from the vector by PvuII digestion, and it possesses a functional promoter and operator as demonstrated by in vivo tests. The 41-bp fragment contains several restriction sites: HincII, TaqI, RsaI, and a HpaI site that is located at the center of the operator sequence. Two new operator derivatives, symmetrical about the HpaI site, were prepared from the 41-bp fragment by joining two right-side, or two left-side PvuII-HpaI pieces together at the HpaI site. These derivatives showed in vivo operator activity. Plasmids containing up to five copies of the 41-bp trp-promoter-operator fragment have been constructed. These plasmids should be useful in preparing large amounts of the 41-bp fragment.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Texas"><name sortKey="Russell, David R" sort="Russell, David R" uniqKey="Russell D" first="David R." last="Russell">David R. Russell</name></region><name sortKey="Bennett, George N" sort="Bennett, George N" uniqKey="Bennett G" first="George N." last="Bennett">George N. Bennett</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003A23 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003A23 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= OperaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:7B3E2C0AC7EACC67BF7C383593CF127FE124EB89
|texte= Cloning of small DNA fragments containing the Escherichia coli tryptophan operon promoter and operator
}}
| This area was generated with Dilib version V0.6.21. |  |